Advanced search

Search results      


Development of a Novel Osteopathic Animal Modality of Inflammatory Bowel Disease: Preliminary Findings and Clinical Implications

Journal: The Journal of the American Osteopathic Association Date: 2014/01, 114(1):Pages: e4-e5. doi: Subito , type of study: animal experiment

Full text    (https://www.degruyter.com/document/doi/10.7556/jom_2014_01.0001/html)

Keywords:

animal experiment [60]
IBD [4]
inflammatory bowel disease [8]
LPT [24]
lymphatic pump technique [41]

Abstract:

Introduction: Osteopathic clinicians have reported some success in the treatment of Inflammatory Bowel Disease (IBD) patients; however, objective evidence-based research exploring mechanisms and efficacy of OMT in IBD is lacking, in part due to the lack of an osteopathic IBD animal model. Therefore, the purpose of these experiments was to establish such a model and lay a foundation for future studies. Preliminary results can be used and expanded upon in future studies to evaluate the safety and efficacy of lymphatic pump techniques (LPT) in IBD, to create treatment regimens and guidelines for the application of LPT, and to test this intervention as an adjunct therapy with currently used pharmacological interventions. Hypothesis: We hypothesize that the application of LPT will reduce signs and severity in experimental colitis in a rat model of IBD, which is induced by dextran sodium sulfate (DSS) polymers in their drinking water. Methods: Colitis in male, Wistar rats, weighing around 275 grams, was induced by replacing normal drinking water with water containing 3.5% DSS for 10 days (days 0-9). Treatment was performed daily on days 3-8, which allowed for 3 days of IBD induction by DSS. To minimize stress to both animals and animal handlers, 2-5% isoflurane gas was administered to LPT or sham rats prior to and during treatment. This was done daily for 6 days (days 3-8). Experiments included an LPT group (DSS+ISO+LPT), a sham group (DSS+ISO+light touch), a disease control group (DSS only), and a healthy control group (no treatment and no DSS). Weights and clinical signs of disease were monitored daily. On day 9, rats were sacrificed. Results: Daily treatment with DSS decreased body weight in both DSS and DSS+ISO groups, with a statistically significant difference to the control group on days 7-9. This decreased weight gain was not due to changes in fluid status, as all groups consumed roughly the same amount of fluid. Daily clinical assessment of general health and inflammation is represented by the Disease Activity Index (DAI). The DAI score increased over time, without any changes between DSS and DSS+ISO groups. Mucosal damage was assessed using the macroscopic inflammatory scoring system. We found a statistically significant increase in colonic tissue damage at days 6 and 9 in DSS and DSS+ISO groups when compared to the control group at respective days. LPT significantly decreased DAI as compared to sham and DSS+ISO controls. Of interest, application of LPT restored the weight of the colon still containing fecal contents similar to levels seen in the healthy control group. In addition, LPT also restored stool consistency similar to what was seen in the healthy control group. This is of significance because decreases in filled colon weight are indicative of colonic hypermotility; thus, colons from animals with severe colitis can be seen to be nearly devoid of fecal contents. These results show that LPT influences colonic motility and effects school consistency, thus applying this treatment in a clinical setting could significantly alleviate the symptoms experienced by patients. Conclusion: The results shown in this presentation confirm that the proposed DSS induction model does in fact present a disease model with clinical measurable variables, such as weight changes and DAI. We further showed that LPT had a significant decrease on disease severity and was able to reverse clinical symptoms of IBD.


Search results      

 
 
 






  • ImpressumLegal noticeDatenschutz


ostlib.de/data_bdkegmwhzfrjsncxvpau



Supported by

OSTLIB recommends