Advanced search


Search results        Search results      Copy URL to E-Mail


Therapeutic Effects of Lymphatic Pump Treatment on Lymphangiogenesis and Inflammatory Cytokines in Lymph Nodes of Rats with Adjuvant-Induced Arthritis

Journal: Journal of Osteopathic Medicine Date: 2021/12, 121(12):Pages: A36-A37. doi: Subito , type of study: animal experiment

Full text    (https://www.degruyter.com/document/doi/10.1515/jom-2021-2000/html)

Keywords:

animal experiment [67]
arthritis [76]
LPT [27]
lymphatic pump technique [43]
lymphatic system [54]

Abstract:

Context: Rheumatoid arthritis (RA) is a chronic inflammatory disease caused by self-reactive lymphocytes, eventually destroying joints. To study this disease and potential treatments, the adjuvant-induced arthritis (AIA) rat model can be used. In RA, there is a collapse of appropriate lymphatic drainage1,2 and an increase in inflammatory cytokines in the lymph nodes.3 The osteopathic treatment, Lymphatic pump treatment (LPT), has the potential to increase lymphatic circulation and decrease inflammation.4 Objective: For this study, we tested the hypothesis that LPT would prevent the collapse of lymphatic drainage and promote lymphatic vessel growth in a rat model of arthritis. Furthermore, we hypothesized that this increased lymphatic movement from LPT would allow for the systemic distribution of pro-inflammatory cytokines to alleviate local inflammation at the inflamed joint. Methods: Experimental rats were immunized with Complete Freund’s adjuvant on day zero. Control rats for this study were non-immunized and non-arthritic. After the onset of adjuvant-induced arthritis, rats received either LPT or sham treatment three times a day for six days. On day 21, the draining popliteal lymph nodes (PLN) were harvested and analyzed for cytokine levels (IL-1β, IL-4, IL-6, IL-17, IFN-γ, TNF-α, VEGF-C) or VEGFR-3 by quantitative RT-PCR. Additionally, immunohistochemistry (IHC) was used to visualize VEGF-C and VEGFR-3 expression in the PLNs. The experiment used thirty-six rats total and all experiments were statistically analyzed initially by a one-way analysis of variance followed by a Tukey post-hoc test to determine if significant differences were identified among the groups. Results: Injected rats successfully developed arthritis as shown through a statistically significant increase in ankle circumference and articular index score (AIS). The LPT group showed a slight decrease in ankle circumference and AIS. No statistical differences were seen among the cytokines analyzed in the draining PLNs when comparing the LPT and sham-treated groups. IHC showed an increase in VEGF-C and VEGFR-3 expression in injected animals compared to non-injected animals, with the PLNs from the LPT group having the highest expression area of VEGF-C and the lowest expression area of VEGFR-3 within the injected treatment groups. Conclusion: The injected LPT group showed a slight reduction in ankle circumference and AIS, suggesting that LPT helped alleviate edema and inflammation. While the cytokines analyzed showed no difference between arthritic treatment groups, it is possible that other cytokines may have been impacted, which can be analyzed in future studies. Additionally, in the PLN, LPT trended towards elevated levels of VEGF-C, an important lymphatic vessel growth factor. Taken together, the data suggest LPT may alleviate inflammation in rats through the elevation of a lymphatic growth factor. Through these results, we have better insight into the effectiveness of LPT in treating chronic inflammatory diseases such as RA with the hopes of applying these techniques to improve patient care.


Search results      Copy URL to E-Mail

 
 
 






  • ImpressumLegal noticeDatenschutz


ostlib.de/data_bstyvceakzmnfhqurxjg



Supported by

OSTLIB recommends