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The Effects of Repeated Lymphatic Pump Treatment on Thoracic Duct Lymph Flux and Its Clinical Application

Journal: The Journal of the American Osteopathic Association Date: 2010/08, 110(8):Pages: 474-475. doi: Subito , type of study: animal experiment

Full text    (https://www.degruyter.com/document/doi/10.7556/jaoa.2010.110.8.445/html)

Keywords:

animal experiment [68]
LPT [27]
lymph flow [4]
lymphatic pump technique [43]
thoracic duct [6]

Abstract:

Background: Lymphatic pump techniques (LPTs) are used by osteopathic practitioners for the treatment of edema and infection; however, the mechanisms by which LPTs enhances the lymphatic and immune systems are poorly understood. Previous studies from our laboratory demonstrate that lymphatic pump treatment (LPT) enhances thoracic duct lymph flow and leukocyte numbers in both dogs and rats. Further, there are inconsistent reports in the literature discussing the optimal duration and repeated application of LPT. This presentation will show a novel scientifically backed approach to LPT. The purpose of this study was to measure the effects of repeated LPT on thoracic duct lymph flow and leukocyte concentrations. Hypothesis: Repeated application of LPT would enhance thoracic duct lymph flow and leukocyte concentrations. Methods: Under anesthesia, the thoracic ducts of 6 dogs were cannulated, lymph flow was measured by timed collection and lymph samples was collected at (1) 30-minute baseline (pre-LPT), (2) during 4 minutes of LPT, (3) continuously for 2 hours following LPT (resting), (4) during 4 minutes of a second application of LPT, and (5) for 30 minutes following the second application of LPT (resting). Leukocytes in samples of thoracic duct lymph were enumerated using the Hemavet 950 (Drew Scientific). Leukocyte flux in the thoracic duct was computed from the product of lymph flow and leukocyte concentration. Results: The baseline leukocyte flux in thoracic duct lymph was 4±1.5 × 106 cells/min and LPT significantly increased their concentration to 14±50 × 106 cells/min. During the 2-hour resting period between LPT treatments, leukocyte numbers were similar to baseline (2.8±0.66 × 106 cells/min). A second application of LPT again increased thoracic duct leukocyte concentrations to 137±17 × 106 cells/min. During the 30 minutes following the second LPT, the thoracic duct leukocyte flux was 5.8±1.7 × 106 cells/min. Conclusions: These results indicate that repeated applications of LPT will transiently enhance thoracic duct leukocyte flux. The information gained from this study provides a rationale for the clinical use of LPT to enhance immunity.


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