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Osteopathic lymphatic techniques reduces cortisol plasma level in rats after repeated treatments

Journal: International Journal of Osteopathic Medicine Date: 2013/03, 16(1):Pages: e19-e20. doi: Subito , type of study: animal experiment

Full text    (https://www.sciencedirect.com/science/article/pii/S1746068913000126)

Keywords:

[7918]

Abstract:

Background Many reports evidence in humans the positive effect of manipulation, in particular of osteopathic lymphatic techniques in normal as well in pathological conditions. In animals too, lymphatic treatment has been reported to induce changes of lymphatic flux and to mobilize inflammatory mediators thus determining positive effects on the immune system. Methods Twelve male Wistar rats (weight 180 g) were randomly allocated to control (6 rats) and experimental (6 rats) group. Experimental group rats underwent lymphatic techniques for 10 min each day for 5 consecutive days under gaseous anaesthesia. Control group animals were anaesthetized but did not have lymphatic treatment. Blood samples were collected before and at the end of the experiment period. A complete blood cell count and serum chemistry were determined. TNF-alpha and IL-1 beta were determined in order to evaluate changes in inflammatory response. Cortisol was also determined as indicative of animal stress. The experiment was carried on in August 2011 and was replicated in February 2012. Results Blood cell count did not show differences at baseline and at the end of the experiment both in control and in treated rats. Blood serum chemistry was also unchanged. In particular, blood urea nitrogen (BUN) and serum creatinine (expression of renal function), alanine transaminase (ALT) and aspartate transaminase (AST) (indicative of hepatic cell integrity) were normal and did not change after lymphatic treatment. No changes in plasmatic level of cytokines were found after the five-days lymphatic treatment. On the contrary and unexpectedly, cortisol level was significantly reduced in rats that were treated with lymphatic techniques. In untreated rat group cortisol level was, however, minimally increased. These findings were confirmed by the results of the replicated study. Conclusions This study demonstrates that repeated lymphatic treatment is a safe procedure. Moreover, lymphatic techniques induce reduction in cortisol level, which may be related to an anti-stress effect.


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