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Abdominal Lymphatic Pump Treatment Increases Leukocyte Count and Flux in Thoracic Duct Lymph

Journal: The Journal of the American Osteopathic Association Date: 2007/08, 107(8):Pages: 355. doi: Subito , type of study: animal experiment

Full text    (https://www.degruyter.com/document/doi/10.7556/jaoa.2007.107.8.327/html)

Keywords:

animal experiment [60]
immune response [11]
leukocytes [8]
LPT [24]
lymphatic pump technique [41]
lymphatic system [47]

Abstract:

The lymphatic pump technique (LPT) was designed to improve lymph flow, reduce edema, and improve the body's immunological defenses to combat infections. Studies suggest that LPT enhance immunity and resistance to infection, but direct evidence of this has not been documented. To test the hypothesis that LPT enhances lymph flow and leukocyte concentrations in lymph, the immediate effect of LPT on lymph flow and leukocyte flux and in the canine thoracic lymph duct was measured. Lymph flow data and lymph samples were collected at baseline and at 2-3 min intervals during LPT. The baseline leukocyte count was 4.8±1.7 million cells/ml, and LPT increased leukocytes to 11.8±3.6 million cells/ml (P<0.05). Whereas numbers of macrophages, neutrophils, total lymphocytes, T cells, B cells, and IgG forming B cells increased similarly during LPT, their relative percentage in lymph was unaltered by LPT; however, IgA antibody forming B cells increased from 5.8% at baseline to 17% during LPT. This data suggest that LPT acts preferentially on mucosal tissues, a potential source of these mobilized leukocytes. Furthermore, LPT enhanced lymph flow approximately 4-fold. Leukocyte flux was computed from the product of lymph flow and cell count, and LPT enhanced leukocyte flux from 8.2±4.1 million leukocytes per min to 60±25 million leukocytes per min. The most important new finding of this investigation was that LPT produced large increases of leukocytes in lymph. Since LPT also increased lymph flow in the thoracic duct, the flux of leukocytes transported to the circulation through the thoracic duct was greatly increased. Furthermore, LPT increased IgA antibody forming B cells, suggesting LPT enhances the mobilization and lymphatic transport of mucosal immune cells during abdominal compression. It is likely that this mobilization of leukocytes is an important mechanism responsible for the increased immune responses of patients treated with LPT. Thus, the results of this investigation may provide a rational basis for the use of LPT to enhance immunological function and treat infection.


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