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The Effects of Lymphatic Pump Manipulation on Tumor Development and Metastasis

Journal: The Journal of the American Osteopathic Association Date: 2009/08, 109(8):Pages: 453. doi: Subito , type of study: animal experiment

Full text    (https://www.degruyter.com/document/doi/10.7556/jaoa.2009.109.8.425/html)

Keywords:

animal experiment [60]
cancer [51]
LPT [24]
lung [33]
lymphatic pump technique [41]
tumor [13]

Abstract:

Hypothesis: It is well recognized in the osteopathic community that metastatic cancer or cancer with metastatic potential is considered a relative or absolute contraindication for certain osteopathic manipulative treatments (OMT), including high velocity low amplitude (HVLA) and lymphatic pump techniques (LPT). However, studies from our laboratory demonstrate that the application of LPT to the rat increases thoracic duct lymph flow and leukocyte numbers, and enhances survival and reduces pulmonary bacteria during pneumonia. In addition to the direct effects of increased numbers of circulating leukocytes produced by LPT, it seems likely that these cells improve immune surveillance, which may enhance protection against tumor development and metastasis. Methods: To test the hypothesis that LPT enhances antitumor immunity, F344 rats were injected intravenously with MADB106 tumors. Twenty four hours following tumor injection, rats received: (1) no treatment (control), (2) 4 minutes of light touch under anesthesia (sham), or (3) 4 minutes of LPT under anesthesia for 5 consecutive days. Seven days after tumor injection, lungs were removed for assessment of tumor metastasis and leukocyte populations. Results: The application of sham and LPT reduced pulmonary tumors by approximately 50% compared to control. In addition, sham and LPT increased pulmonary leukocytes approximately twofold compared to control. This finding suggests that the administration of isoflurane anesthesia during either sham or LPT increases leukocyte trafficking into lungs and subsequent tumor killing. Therefore, to ascertain if the application of sham or LPT under isoflurane anesthesia would increase the pulmonary trafficking of leukocytes in the absence of tumors, healthy rats were given control, sham, or LPT as described. The administration of isoflurane increased pulmonary leukocyte numbers approximately twofold, suggesting that pulmonary tumors are not necessary to increase leukocyte trafficking into lungs in response to isoflurane anesthesia. Conclusion: The results from our preliminary studies demonstrate that the administration of isoflurane gas (during either sham or LPT) increases leukocyte trafficking into healthy lungs and lungs burdened with tumors. In addition, the use isoflurane gas during sham or LPT enhanced killing of tumors in the lung. In our ongoing studies, we are exploring new anesthetics to elucidate the effects of LPT on tumor development and metastasis.


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