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Lymphatic Pump Treatment of Rats With Adjuvant-Induced Arthritis

Journal: The Journal of the American Osteopathic Association Date: 2011/08, 111(8):Pages: 507-508. doi: Subito , type of study: animal experiment

Full text    (https://www.degruyter.com/document/doi/10.7556/jaoa.2011.111.8.487/html)

Keywords:

animal experiment [67]
LPT [27]
lymphatic pump technique [43]
rheumatoid arthritis [7]

Abstract:

Background: Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease characterized by synovial inflammation and lymphocyte infiltration. Lymphatic pump treatment (LPT) is clinically used to reduce edema and aid the immune response through increasing lymphatic circulation. Purpose: This study examined the effect of LPT on arthritis using the rat adjuvant-induced arthritis (AIA) model of RA. We hypothesized that LPT of rats with established arthritis would result in reduced inflammation through increased lymphatic circulation resulting in T regulatory cell (Treg) function. Methods: AIA was initiated in 12 female Lewis rats by subcutaneous injection of killed Mycobacterium butyricum above the base of the tail. Arthritic rats were randomly placed in 2 groups; the LPT group and the sham group which was held. LPT was performed 6 times over 11 days without anesthesia and utilized a diaphragmatic approach generating a pulsation in the fluids and tissues by rhythmically pressing below the rib cage 30 to 40 times over 30 seconds. Arthritis was assessed by measuring ankle circumferences and by articular index scoring. Blood smears and flow cytometry were performed to study leukocyte populations. Results: The LPT rats trended to have less swollen ankles throughout the usual peak of arthritis, which was greatest on day 21 when the ankle circumferences were 3.1 mm smaller (∼40% less) than the sham-treated animals. The LPT rats also trended to have lower articular index scores. During the peak of inflammation, the LPT animals had an articular index score of 1.75 compared to sham-treated animals score of 2.17. Overall, the articular index scores of the LPT animals seemed to plateau while the articular index scores of the sham-treated animals continued to rise. The LPT group had a higher percentage of CD3+ cells than the sham group (76.3% to 67.8%). This suggests that more lymphocytes were circulating in the blood with LPT. Also, the percentage of peripheral blood Treg cells (CD4+, CD25+) were increased with LPT compared to the sham group (0.7% to 0.51%). Conclusion: These results suggest that the progression of arthritis can be blunted with LPT. Specifically, analysis of ankle circumference (a direct measure of edema) and articular index score (a subjective measure of inflammation) suggests that LPT resulted in reduced inflammation particularly during the peak inflammatory period of AIA. The LPT may be accomplishing this through increasing lymphocyte circulation.


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