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The Effects of Osteopathic Manipulation Techniques on the Lymphatic System

Journal: Unpublished MSc thesis University of North Texas Health Science Center, Date: 2021/04, Pages: 58, type of study: animal experiment

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Keywords:

animal experiment [36]
inflammation [22]
lymphatic pump technique [21]
lymphatic system [40]
pilot study [104]

Abstract:

Purpose: Osteopathic manipulation techniques (OMT) have been utilized by osteopathic physicians to treat a variety of conditions including musculoskeletal dysfunctions, edema, inflammation, and disease. However, the mechanisms by which OMT aides the body in healing are not well understood. The long-term goal of our research is to advance our understanding of the impact of OMT on inflammatory disease, such as sepsis. The purpose of this study was to develop a swine model that will be used in future studies investigating the impact of OMT on the lymphatic system during disease. We hypothesized that the abdominal lymphatic pump technique (LPT) would enhance thoracic duct lymph (TDL) flow. Methods: Four swine (two male and two female) were placed under anesthesia. The thoracic duct was exposed via thoracotomy then cannulated using an angio-catheter. TDL was collected during 4-min baseline, 4-min LPT, and 4-min post-LPT. TDL flow was measured by timed collection during each phase. TDL was centrifuged to remove the cellular components, and the supernatant was stored for biomarker analysis. Results: TDL flow at baseline was 2.2 ± 1.0 mL/min and LPT increased lymph flow rate to 5.6 ± 1.8 mL/min. In two experiments, thoracic lymph nodes and thoracic duct lymph were collected, and leukocytes were isolated to optimize the flow cytometry staining protocol. Conclusion: In our pilot study, LPT increased TDL flow 2.5-fold and demonstrated surgical feasibility. In future studies we will study the physiological effects of OMT, including LPT, during sepsis. This knowledge would provide an evidence-based foundation for the use, or contraindication, of OMT during sepsis and inform therapeutic decisions of osteopathic physicians.


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