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The Development of a Rodent Model to Study the Effects of Lymphatic Pump Treatment on the Lymphatic and Immune System

Journal: The Journal of the American Osteopathic Association Date: 2009/08, 109(8):Pages: 452. doi: Subito , type of study: animal experiment

Full text    (https://www.degruyter.com/document/doi/10.7556/jaoa.2009.109.8.425/html)

Keywords:

animal experiment [60]
immune system [43]
LPT [24]
lymphatic pump technique [41]
lymphatic system [47]

Abstract:

Hypothesis: Osteopathic physicians have long recognized the importance of the lymphatic system in the maintenance of health and have included treatments such as the lymphatic pump technique (LPT) in clinical trials studying the effects of manipulative medicine on infectious disease with promising results. However, there has been a lack of animal research in this field. Previously our laboratory developed a large animal model to study LPT in healthy subjects. In the canine model, LPT increased lymph flow, leukocyte concentrations, and cytokine release from both the thoracic and intestinal lymph duct. In order to study the effects of LPT in a disease model, we have developed a rodent model of LPT, which allows us to use better characterized disease models, have access to a wider range of reagents and biomarkers, and minimize cost. Methods: In order to demonstrate that LPT in our rodent model is comparable to the canine model in an acute setting, a catheter was inserted into the thoracic duct of the rats and lymph was collected during baseline, 4 minutes of LPT, and recovery. In addition, we collected blood following a single application of LPT. In contrast to our large animal model, the repeated application of LPT requires the use of anesthesia. Due to this difference, we have characterized the use of volatile and nonvolatile anesthetics in healthy animals in order to perform repeated treatments over several days. Results: In the thoracic duct lymph, both lymph flow and leukocyte counts were increased, resulting in a greater than threefold increase in leukocyte flux through the thoracic duct. An increase of 4,000,000 leukocytes was seen in the jugular venous blood approximately 45 minutes following treatment. We also found that the daily application of the inhaled volatile anesthetic isoflurane caused a significant increase in lung leukocytes after 6 days, regardless of disease status. We replaced isoflurane with propofol, a nonvolatile, shorter acting sedative in order to minimize immune modulation in the tissue sites of our disease models. Conclusion: Following the development and characterization of the rodent model of LPT, we will be able to study the effects of LPT on both a pneumonia and metastatic cancer, both of which have significant clinical relevance in the osteopathic community.


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