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Effect of Occipito-Atlantal Decompression, Transcutaneous Auricular Vagus Nerve Stimulation, and the Splenic Pump on Circulatory Immune Cell Numbers

Journal: The AAO Journal Date: 2023/06, 33(2):Pages: 37. doi: Subito , type of study: controlled clinical trial

Full text    (https://meridian.allenpress.com/aaoj/article/33/2/20/493544/LBORC-NUFA-Poster-Abstracts-2023-Students)

Keywords:

blood system [2]
controlled clinical trial [283]
immune system [40]
nerve stimulation [2]
occipito-atlantal decompression [1]
OMT [2951]
osteopathic manipulative treatment [2973]

Abstract:

Introduction/Background: Occipito-atlantal decompression (OA-D) and transcutaneous auricular vagus nerve stimulation (taVNS) may activate the cholinergic anti-inflammatory pathway through parasympathetic activation. The splenic pump may augment this effect on immune function. Objective: The objective is to evaluate the effects of OA-D or taVNS combined with the splenic pump on circulatory immune cell numbers. Methods: Subjects (n=34), healthy adults of both genders, met inclusion criteria. OA-D or taVNS was performed on three consecutive days followed by either the splenic pump or a sham intervention. Blood was drawn on the third day. OA-D (fingers placed at the level of the OA, applying 5 N for 10 minutes), taVNS (current of 2 mA, 10 Hz, 300 ųs pulse width applied via a bipolar clip electrode attached the ear for 10 minutes), splenic pump technique (rhythmic pumping of the left upper abdomen at 2-4 mmHg and 30 compressions/minute for 10 minutes) were performed. Leukocytes were detected using fluorescence-labeled antibodies, analyzed using multiple linear regression analysis with the R Statistical Platform. Dependent variables: relative percentage of total immune cells. Independent variables: body mass index (BMI) and whether OA-D, taVNS, or the splenic pump had been performed. Independent parameters: significant at P<0.05, trends at P<0.15. Results: B-cells were reduced by the splenic pump (-0.21, P<0.15). Splenic pump increased monocytes (+0.51, P<0.15),OA-D decreased monocytes (-0.74, P<0.15). Splenic pump decreased NK cells (-0.71, P<0.05). Discussion/Conclusion: OA-D decreased monocytes which was offset by the splenic pump. The splenic pump markedly reduced NK cells and mobilized monocytes from the spleen. This suggests translocation into the systemic circulation, with an exchange of immune cells between the spleen and the blood. Study limit: we cannot determine where cells are being translocated.


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