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Amelioration of Oxidative Stress and Motor Deficits With Osteopathic Manipulative Treatment in a Mouse Model for Human Parkinson Disease

Journal: The Journal of the American Osteopathic Association Date: 2009/08, 109(8):Pages: 428. doi: Subito , type of study: animal experiment


Keywords:

animal experiment [67]
OMT [3854]
osteopathic manipulative treatment [3875]

Abstract:

Hypothesis: Pitx-aphakia (ak) mice closely resemble the neuropathology, age-related progression, and behavioral consequences associated with Parkinson disease (PD) in humans. PD-related oxidative stress is linked to augmented urinary levels of the DNA-damage biomarker 8-hydroxydeoxyguanosine (8-OHdG) in humans. We hypothesize that ak mice mimic PD-related oxidative stress resulting in elevated urinary 8-OHdG. We further hypothesize that modified osteopathic manipulative treatment (OMT) of ak mice will significantly lower urinary 8-OHdG levels through improved fine motor coordination and rigidity. Materials and Methods: To establish a urinary baseline of 8-OHdG in ak versus healthy control animals (strain C57BL/6J), 11am urine samples were analyzed for 8-OHdG and creatinine (glomerular filtration rate) using an enzyme-linked immunosorbent assay (ELISA). Subsequently, ak animals were randomly divided into an OMT treatment group receiving modified muscle energy using the active direct technique and a control group receiving a sham procedure to the corresponding body regions. To eliminate individual variability, all procedures were performed by the same, trained individual under professional supervision. Treatments were conducted as 1-minute sessions, each repeated three times. Urinary levels of 8-OHdG and creatinine were assessed before and after OMT/sham procedures. Lastly, all subjects underwent motor coordination and muscle rigidity “hourglass” testing. Urinary 8-OHdG/creatinine, motor skills competence, and rigidity were scored and statistically analyzed (t test, P≤.05). Results: Urinary 8-OHdG was significantly elevated in ak mice compared to healthy controls. There was however, no relevant difference in urinary 8-OHdG resulting from OMT treatment. OMT-treated ak mice performed significantly better in the rigidity testing than sham-treated controls, whereas no difference in motor coordination competence could be observed between the two groups. Conclusion: We were able to establish 8-OHdG as a urinary biomarker for PD-related oxidative stress in ak mice. The antioxidant effect of treatment modalities and dietary supplements in respect to PD can now be conveniently tested in an animal model of the disease. OMT was effective in the improvement of muscle rigidity symptoms associated with PD. Studies that are more detailed may be necessary to link OMT to reduced systemic oxidative stress and improved motor coordination in PD.


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