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Acute Effects of Osteopathic Manipulative Treatment on Persistent Hindlimb Flexion

Journal: The Journal of the American Osteopathic Association Date: 2012/08, 112(8):Pages: 534. doi: Subito , type of study: animal experiment

Full text    (https://www.degruyter.com/document/doi/10.7556/jaoa.2012.112.8.529/html)

Keywords:

animal experiment [67]
hindlimb [1]
flexion [32]
OMT [3746]
osteopathic manipulative treatment [3766]

Abstract:

Hypothesis: Persistent hindlimb flexion (PHF) that lasts for weeks is induced by noxious electrical stimulation to the mid-thigh in the anesthetized rat. It serves as an animal model for restricted range of motion associated with somatic dysfunction. In this study, we investigated the effects of osteopathic manipulative treatment (OMT) on PHF. Our null hypothesis was that OMT would not affect PHF. Methods: First, we evaluated the effect of acute OMT on PHF. We induced PHF in anesthetized rats by delivering electrical stimulation (2 mA pulses, 7 ms, 100 Hz) across wound clips applied to the medial and lateral thigh for 1 hour. The initial PHF was measured by applying weight in grams (g) to the flexed limb until leg lengths were equal. Then, a single OMT session (eg, soft tissue, balanced ligamentous tension, facilitated positional release technique, stretching) was performed by 1 of 2 practitioners for 7 minutes and PHF was remeasured. During sham treatment (SHAM) the practitioners' hands rested lightly on the caudal half of the rat for 7 minutes. Second, we evaluated the effect of OMT delivered on day 0 and remeasured 3 days later. Third, we evaluated the effect of repeated OMT on consecutive days. Persistent hindlimb flexion was measured prior to and after a single OMT session on days 0, 1, and 2. Results: A 2-way analysis of variance revealed a significant treatment effect (SHAM vs OMT, F1,12=12.27, P=.004) but no practitioner (F1,12=1.4, P=.250) or interaction effects (F1,12=3.19, P=.099) on day 0. The OMT group experienced a larger reduction in PHF, as measured by mean (standard deviation) (7.4 g [1.13 g]), than the SHAM group (2.4 g [0.70 g]). A single initial OMT session did not affect PHF remeasured on day 3 (t test, P<.617). Repeated OMT resulted in a trend toward acute effects on days 1 and 2, when reductions for the SHAM group ranged from -4.4 g to 4 g and reductions for the OMT group ranged from 0 g to 11 g. Conclusion: A single, brief OMT session reduced PHF immediately after induction, and this effect was not dependent on the practitioner. The single OMT effect did not last when measured 3 days later. There was a trend toward acute OMT effects when administered on subsequent days. Future investigations will evaluate the effects of longer-duration OMT with and without increased frequency.


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