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Initial Investigation Into The Effect of Nerve Compression Syndrome on Slow-Twitch Muscle, and Treatment With Osteopathic Manipulative Therapy (OMT)

Journal: Journal of Osteopathic Medicine Date: 2004/08, 104(8):Pages: 340. doi: Subito , type of study: animal experiment

Full text    (https://www.degruyter.com/document/doi/10.7556/jaoa.2004.104.8.337/html)

Keywords:

animal experiment [67]
nerve compression syndrome [4]
OMT [3746]
osteopathic manipulative treatment [3766]
palliative care [19]

Abstract:

As part of the continuing investigation of the effects of nerve compression syndromes (NCS) on muscle function, we have initiated an assessment of the effects of NCS, and of palliative OMT, on the function of single slow-twtich (soleus) muscle cells. Methods involved uniliteral compression of the rat sciatic nerve (including piriformis syndrome) produecd by suture ligation at either 100 or 60 mm Hg, at the level of the obturator tendon, with one group receiving no treatment and one group having three-times weekly OMT of the hindlimb using the techniques of Hallas, et al. (1997 JAOA 97:207-214). Animals were euthanized at four, eight and twelve weeks post-ligation, the soleus removed and chemically demembranated (0.5% Triton X-100 in control solution, and single fibers dissected out. All experiments were run at 22 degrees Celsius, pH7, with solutions formulated by solving the set of simultaneous equations describing the multiple equilibria of ions in solution. Solutions contained (mM): 5 EGTA, 20 imidazole, 2 Mg, 5 MgATP, 15 phosphocreatine and 100 u/ml creatine kinase (to maintain a constant [ATP]). All solutions had a total ionic strength of 200 mM. Appropriate Ca was added to assure attainment of intermediate and maximal force (Fmax) under all conditions, and KH2PO4 (Pi) was added as required to assess sensitivity of soleus muscle fibers to Pi, the primary fatigue metabolite in muscle, used here and previously as a marker of the functional change of muscle. Results indicate that atrophy of the cells occured, and the sensitivity of soleus fibers to Pi was increased (less force generated) in direct proportion to duration of ligation and to compression tension. In the later case the degress of change was significantly greater than seen in extensor digitorum longus fast-twitch muscle fibers previously reported. Results also indicated no significant change in Ca sensitivity of soleus fibers following NCS. Furthermore, under all conditions (but not in all cases), there was a tendency for amelioration of the atrophy and the Pi sensitivizing effect if palliative hindlimb OMT was used. These results provide further evidence of the specific changes occuring in muscle suffering NCS, and that such alterations may be ameliorated by OMT.


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